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取不同头臀长(CRL)10～22mm(E13～18)鼠胚腹侧中脑制成悬液,分别移植至帕金森氏病模型鼠去多巴胺(DA)神经侧纹状体中。发现用CRL为10～16mm(E13～15)胚脑为供体的受移植鼠行为效应和移植区DA神经元存活情况远较以CRL为17～22mm供体为佳。用酪氨酸羟化酶(TH)免疫组化ABC法观察了不同胚龄(E_(13)～P_0)黑质纹体系DA神经元的形态和分布,发现CRL10～16mm(E_(13～15))时TH阳性细胞位于Sylvius导水管腹侧,此时DA
The ventral midbrain of rat embryos with different head buttocks length (CRL) 10-22mm (E13-18) was taken as a suspension and transplanted into the dopaminergic (DA) neurolateral striatum of model rats with Parkinson’s disease. It was found that the behavioral effects of transplanted mice with CRLs of 10-16 mm (E13-15) embryonic brain donors and survival of DA neurons in the transplanted region were better than those with 17-22 mm donors with CRL. Using tyrosine hydroxylase (TH) immunohistochemical ABC method to observe the morphology and distribution of DA neurons in the niribrid system of different embryonic ages (E_(13)～P_0) and found that CRL 10~16mm(E_(13~15 )) TH positive cells ventral to the Sylvius aqueduct at this time DA