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目的:阐明NO对大鼠生精能力的影响及其作用机制。方法:大鼠去垂体,观察硝普钠(SNP)与N-ω单硝基L-精氨酸甲酯(L-NAME)对大鼠生精功能的直接作用。用放射免疫法测定血清内卵泡刺激素(FSH)、黄体生成素(LH)及睾酮含量;Greiss法测定血清NO-x含量;流式细胞术测定各生精细胞DNA含量,计算1c、2c及4c期细胞的百分比。结果:各组血清LH、FSH和睾酮浓度显著下降或逐渐下降。SNP组血清内NO-x含量显著升高,L-NAME组血清内NO-x含量显著降低;对照组、L-NAME组和SNP+LNAME混合注射组的细胞分布均以1c为主;而SNP组的细胞以2c为主。结论:体内高浓度的糖皮质激素可屏蔽LH和FSH的分泌,进一步抑制睾酮的分泌;SNP与LNAME可通过NO直接影响生精细胞的凋亡。
Objective: To clarify the effect of NO on spermatogenesis in rats and its mechanism. Methods: The rats were devided into pituitary to observe the direct effect of sodium nitroprusside (SNP) and N-omega-L-arginine methyl ester (L-NAME) on spermatogenesis in rats. Serum follicle stimulating hormone (FSH), luteinizing hormone (LH) and testosterone were measured by radioimmunoassay. Serum NO-x content was determined by Greiss method. DNA content of each spermatogenic cells was determined by flow cytometry. 4c cells in the percentage. Results: The levels of serum LH, FSH and testosterone in each group were significantly decreased or decreased gradually. The content of NO-x in the serum of SNP group was significantly higher than that in the L-NAME group, while the level of NO-x in the L-NAME group was significantly lower than that in the control group, L-NAME group and SNP + LNAME mixed injection group Group 2c-based cells. Conclusion: High concentrations of glucocorticoids in vivo can shield the secretion of LH and FSH and further inhibit the secretion of testosterone. SNP and LNAME can directly affect the apoptosis of spermatogenic cells through NO.