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通过对转 Bt杀虫蛋白基因油菜植株的后代进行卡那霉素抗性分析和 PCR技术检测 ,结果表明 :Bt杀虫蛋白基因是以单拷贝、杂合地整合到转基因植株 (T0 1、T0 2、T0 3、T0 5、T0 6、T0 7、T0 8)的基因组中 ,并稳定地遗传。EL ISA检测表明 :在第 3～第 9叶期 Bt杀虫蛋白基因在转基因油菜植株中高效地表达 ,Bt杀虫蛋白的表达量为 170～ 2 6 0 ng/ 2 5mg鲜叶 ,占植物可溶性蛋白的 0 .0 6 7%～ 0 .10 5 % ,其抗虫效果高达 72 .0 %～ 94 .4 %。但从第 11叶期后 Bt杀虫蛋白表达量显著地降低 ,只有 7～ 5 0 ng/ 2 5 mg鲜叶 ,占植物可溶性蛋白的 0 .0 0 3%～ 0 .0 2 %。
The results showed that the Bt insecticidal protein genes were homozygously integrated into the transgenic plants (T0 1, T0) by PCR analysis of the offspring of transgenic Bt insecticidal rapeseed plants. 2, T0 3, T0 5, T0 6, T0 7, T0 8) and was stably inherited. The result of ELISA showed that the Bt insecticidal protein gene was highly expressed in the transgenic plants at the 3rd to 9th leaf stage. The expression level of Bt insecticidal protein was 170 ~ 260 ng / 25 mg fresh leaves, accounting for the plant soluble The protein 0 0 6 7% ~ 0 10 5%, the insect resistance as high as 72 .0% ~ 94 4%. However, the expression level of Bt insecticidal protein decreased significantly after the 11th leaf stage, with only 7 ~ 500 ng / 25 mg fresh leaves, accounting for 0.003% -0.02% of the plant soluble protein.