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目的:探讨硫化氢(H2S)后处理对H9C2细胞缺氧/复氧损伤的保护作用及其机制。方法:细胞随机分为:正常对照组(con)、缺氧/复氧组(H/R)、NaHS后处理组(H/R+NaHS)、CSE阻断剂干预组(H/R+PAG)。倒置显微镜、流式细胞仪检测细胞凋亡;实时荧光定量PCR检测胱硫醚-!-裂解酶(CSE)mRNA表达;Western blotting检测p50ATF6、GRP78蛋白表达。结果:H/R组CSE mRNA表达较对照组明显降低(P<0.01);H/R+NaHS组CSE mRNA表达较H/R组明显升高(P<0.01)。H/R组p50ATF6、GRP78表达较对照组明显升高(P<0.01);NaHS后处理后p50ATF6和GRP78表达较H/R组升高(P<0.05);给予PAG后,p50ATF6、GRP78表达较H/R组降低(P<0.01)。结论:H2S后处理对H9C2细胞缺氧/复氧损伤具有保护作用,其机制可能与H2S可活化ATF6,上调GRP78表达,降低内质网应激有关。
Objective: To investigate the protective effect and its mechanism of hydrogen sulfide (H2S) postconditioning on hypoxic / reoxygenation injury in H9C2 cells. Methods: The cells were randomly divided into three groups: control group, hypoxia / reoxygenation group (H / R), NaHS postconditioning group (H / R + NaHS), CSE blocker intervention group ). The apoptosis of cells was detected by inverted microscope and flow cytometry. The mRNA expression of cystathionine-cleaving enzyme (CSE) was detected by real-time fluorescence quantitative PCR. The protein expressions of p50ATF6 and GRP78 were detected by Western blotting. Results: The expression of CSE mRNA in H / R group was significantly lower than that in control group (P <0.01). The expression of CSE mRNA in H / R + NaHS group was significantly higher than that in H / R group (P <0.01). The expression of p50ATF6 and GRP78 in H / R group was significantly higher than that in control group (P <0.01); the expression of p50ATF6 and GRP78 in NaHS group was higher than that in H / R group (P <0.05) H / R group decreased (P <0.01). CONCLUSION: H2S can protect H9C2 cells against hypoxia / reoxygenation injury. The mechanism may be related to the fact that H2S can activate ATF6, up-regulate the expression of GRP78 and decrease the endoplasmic reticulum stress.