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目的:了解米非司酮对绒毛组织中MTA3(Metastasis-associatedgene3)的表达及对雌、孕激素受体的影响。方法:对120例停经49天以内临床证实为早期妊娠,要求终止妊娠的妇女,根据停经天数的不同分为停经45天以下及45～49天两大组,每组60例,各组随机分为3小组,分别予米非司酮100mg,200mg,以及对照组。应用RT-PCR法检测各组MTA3的表达。并从各组中各自随机挑选5例,用ERα、PR单克隆抗体免疫组织化学法检测绒毛组织的ERα、PR水平。结果:4组用米非司酮的早孕妇女绒毛中MTA3表达均较对照组增高,差异有统计学意义(P<0.05),并表现为剂量相关性,随着米非司酮的剂量增加,MTA3的表达增高。服药组ERα水平增高,阳性物质定位于胞浆中,胞核中阴性。服药组PR水平低于对照组。结论:米非司酮用于早孕妇女可能通过直接或间接的途径影响雌激素通路,导致滋养细胞m-RNA水平MTA3表达增多,从而有可能使一些细胞黏附分子丢失而导致滋养细胞的侵袭能力的改变,达到抗早孕目的。
Objective: To investigate the effect of mifepristone on the expression of MTA3 (Metastasis-associated gene 3) and its effect on estrogen and progesterone receptors in villi. Methods: 120 cases of menopause within 49 days clinically confirmed as early pregnancy, requiring termination of pregnancy women, according to the number of menopause different menopausal 45 days and 45 days and 45 days 49 days two major groups, 60 cases in each group randomized Three groups were given mifepristone 100mg, 200mg, and the control group. The expression of MTA3 in each group was detected by RT-PCR. Five patients were randomly selected from each group. ERα and PR levels were determined by immunohistochemistry of ERα and PR monoclonal antibodies. Results: Compared with the control group, the expression of MTA3 in the fuzz of 4 pregnant women with mifepristone was significantly higher than that of the control group (P <0.05), and showed the dose-dependent. With the increase of the dose of mifepristone, MTA3 expression increased. The medication group ERα levels increased positive substances located in the cytoplasm, the nucleus negative. The level of PR in the medication group was lower than that in the control group. Conclusion: Mifepristone used in early pregnant women may directly or indirectly affect the estrogen pathway, resulting in increased m-RNA level of trophoblast MTA3 expression, which may make some cell adhesion molecules lost and lead to trophoblast invasion Change, to achieve the purpose of anti-pregnancy.