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目的:研究人参水提物对H_2O_2损伤的H9c2心肌细胞的保护作用。方法:以100μmol/L H_2O_2诱导H9c2心肌细胞建立氧化应激损伤模型,人参水提物在建模前12h加入,共培养24h后,分别用MTT法检测细胞增殖活力,通过比色法、WST-1法和TBA法分别检测培养液上清液中乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果:H_2O_2处理后,细胞存活力降低为原来的48.7%,当生药浓度为0.1mg/m L时人参水提物能使得细胞得到保护,LDH漏出量降低至19.3%,MDA含量降低了23.1%;当生药浓度>1.0mg/m L时,与模型组比较,加药组使得细胞活力提高1.5倍以上,LDH漏出量最多降低98%,同时细胞内的SOD活性最多升高为模型组的2倍以上,MDA含量最多降低61.5%(P<0.05,P<0.01)。结论:人参水提物对H_2O_2损伤的H9c2心肌细胞具有保护作用,有望开发为人参生物活性评价的方法之一。
Objective: To study the protective effect of Ginseng aqueous extract on H9c2 cardiomyocytes injured by H_2O_2. Methods: Oxidative stress injury model was established by inducing H9c2 cardiomyocytes with 100μmol / L H 2 O 2. The aqueous extract of ginseng was added 12h before modeling. After co-cultured for 24h, MTT assay was used to detect the cell proliferation activity. The colorimetric method, WST- 1 method and TBA method were used to detect the contents of lactate dehydrogenase (LDH), superoxide dismutase (SOD) and malondialdehyde (MDA) in the culture supernatant respectively. Results: After H_2O_2 treatment, the cell viability was reduced to 48.7% of the original value. When the crude drug concentration was 0.1 mg / m L, the water extract of Panax ginseng could protect the cells, the LDH leakage decreased to 19.3% and the MDA content decreased by 23.1% ; When the crude drug concentration> 1.0mg / m L, compared with the model group, the drug group increased the cell viability by 1.5 times or more, the LDH leakage decreased by up to 98%, and the intracellular SOD activity increased up to the model group 2 Fold, MDA content decreased by 61.5% (P <0.05, P <0.01). CONCLUSION: Ginseng water extract can protect H9c2 cardiomyocytes injured by H2O2 and is expected to be developed as one of the methods for evaluating the biological activity of Ginseng.