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目的：研究血小板源生长因子（ＰＤＧＦ）对培养的人主动脉平滑肌细胞增殖、胶原蛋白合成、分泌及Ⅰ、Ⅲ型前胶原信使核糖核酸（ｍＲＮＡ）表达和转移生长因子βｍＲＮＡ表达的调节作用。方法：氚胸腺嘧啶脱氧核苷（３ＨＴｄＲ），氚脯氨酸参入及Ｎｏｒｔｈｅｒｎ杂交分析。３ＨＴｄＲ，氚脯氨酸参入的比较采用ｔ检验。结果：ＰＤＧＦ能促进人主动脉平滑肌细胞脱氧核糖核酸（ＤＮＡ）合成和胶原蛋白合成、分泌；ＰＤＧＦ能上调Ⅰ、Ⅲ型前胶原ｍＲＮＡ表达和转移生长因子βｍＲＮＡ表达。结论：血小板源生长因子能通过上调Ⅰ、Ⅲ型前胶原ｍＲＮＡ表达和转移生长因子βｍＲＮＡ表达促进平滑肌细胞胶原蛋白的合成、分泌。
AIM: To investigate the regulatory effect of platelet-derived growth factor (PDGF) on proliferation, collagen synthesis and secretion of cultured human aortic smooth muscle cells and expression of type Ⅰ and Ⅲ procollagen messenger RNA (mRNA) and transforming growth factor-β mRNA. Methods: Tritiated thymidine (3H-TdR), tritiated proline incorporation and Northern blot analysis. 3H TdR, tritiated proline into the comparison using t test. Results: PDGF could promote DNA synthesis and collagen synthesis and secretion in human aortic smooth muscle cells. PDGF could up-regulate the expression of procollagen type Ⅰ and Ⅲ and the expression of metastasis-transforming growth factor-β mRNA. CONCLUSION: Platelet derived growth factor can promote the synthesis and secretion of collagen in smooth muscle cells by up-regulating the expression of type Ⅰ and Ⅲ procollagen mRNA and transforming growth factor-β mRNA.