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转基因动物研究发展至今，对于所构建的表达载体有效性如何，一直缺乏一个验证体系。由于转基因动物的建立较为繁琐复杂，投资大，因而直接用转基因动物去验证所用元件是不明智的。为此，我们采用小鼠乳清酸蛋白（ＷＡＰ）基因（２．６Ｋｂ）为调控序列，人ＧＣＳＦ基因组基因为目的片段，将ＷＡＰ笫一内含子置于ＧＣＳＦ基因５’端，构建成转基因动物乳腺表达载体ｐＩＮＧＧ（Ｆｉｇ．１）。将表达质粒直接注射到怀孕小鼠乳腺。于分婉第８天取乳汁进行ＥＬＩＳＡ检测。在泌乳期小鼠乳汁中检测出人ＧＣＳＦ，表达量达１５３２ｎｇ／ｍＬ（Ｔａｂｌｅ１）。具有一定应用前景。实验中发现：增加注射次数可以提供转染效率，但最佳注射次数仍需摸索。另外，如果换用复制型载体，整合与表达效率有可能大大提高。
Until now, there has been a lack of verification system for the effectiveness of the constructed expression vector. Since the establishment of transgenic animals is more complicated and costly, it is unwise to directly verify the components used by transgenic animals. To this end, we use the mouse whey protein (WAP) gene (2.6Kb) as a regulatory sequence, human G CSF gene for the purpose of the gene fragment, the WAP Zi intron placed G 5 ’end of the CSF gene , Constructed into transgenic animal mammary gland expression vector pINGG (Fig.1). The expression plasmid was injected directly into the pregnant mouse mammary gland. On the 8th day of polydatin, milk was taken for ELISA test. G-CSF was detected in the milk of lactating mice, and the expression reached 1532 ng / mL (Table 1). Has a certain application prospects. The experiment found that: increasing the number of injections can provide transfection efficiency, but the best number of injections still need to explore. In addition, if the switch to copy-type vector, integration and expression efficiency may be greatly enhanced.