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目的:探讨持续游泳运动对D-半乳糖衰老大鼠学习、记忆以及IL-6、IL-10表达的影响。方法:采用D-半乳糖衰老模型,将雄性SD大鼠分为3组:(1)生理盐水对照组(n=10),腹腔注射生理盐水;(2)D-半乳糖安静组(n=10),腹腔注射D-半乳糖(100 mg/kg/d),连续8周;(3)D-半乳糖运动组(n=10),腹腔注射D-半乳糖,并进行持续游泳训练12周。采用Morris水迷宫实验评价大鼠学习记忆能力;免疫荧光染色和蛋白免疫印迹(Western-blotting)检测下丘脑白细胞介素6(IL-6)和白细胞介素10(IL-10)表达。结果:(1)Morris水迷宫实验结果显示,D-半乳糖安静组大鼠寻找平台潜伏期的时间较生理盐水对照组明显延长,在目标象限游泳时间百分比(29.42±2.12)显著低于生理盐水对照组(45.12±1.26)(P<0.05);D-半乳糖运动组潜伏期时间显著短于D-半乳糖安静组(P<0.05),目标象限游泳时间所占百分比(35.35±1.54)较D-半乳糖安静组增加。(2)IL-6免疫荧光染色和蛋白免疫印迹检测结果表明,D-半乳糖安静组IL-6表达较生理盐水对照组显著增加(P<0.05);D-半乳糖运动组IL-6阳性细胞数(8.32±0.43)较D-半乳糖安静组(11.54±0.52)显著减少(P<0.05),表明持续游泳运动有助于降低衰老模型大鼠促炎因子表达水平,抑制炎症反应。(3)IL-10免疫荧光染色和蛋白免疫印迹检测结果表明,D-半乳糖运动组IL-10阳性细胞数(11.22±0.53)较D-半乳糖安静组显著增加(P<0.05),表明持续游泳运动可上调抗炎因子表达水平。结论:持续游泳运动有助于改善衰老大鼠学习记忆能力,并可能通过抑制下丘脑炎症因子IL-6的表达,并提高抗炎因子IL-10的含量,抑制炎症反应,从而延缓衰老。
Objective: To investigate the effects of sustained swimming exercise on learning and memory, IL-6 and IL-10 expression in D-galactose-induced senile rats. Methods: Male Sprague-Dawley rats were divided into 3 groups: (1) saline control group (n = 10), intraperitoneal injection of normal saline; (2) D-galactose sedation group 10), intraperitoneal injection of D-galactose (100 mg / kg / d) for 8 weeks, (3) D-galactose exercise group (n = 10), intraperitoneal injection of D-galactose and continuous swimming training week. The Morris water maze test was used to evaluate the learning and memory ability of rats. The expression of interleukin 6 (IL-6) and interleukin 10 (IL-10) in the hypothalamus were detected by immunofluorescence staining and Western-blotting. Results: (1) The results of Morris water maze test showed that latency of looking for platform in D-galactose quiescent group was significantly longer than that of saline control group, and swimming time percentage in target quadrant was significantly lower than that of saline control group (29.42 ± 2.12) (45.12 ± 1.26) (P <0.05). The latency of D-galactose exercise group was significantly shorter than that of D-galactose sedation group (P <0.05). The percentage of target quadrant swimming time (35.35 ± 1.54) Galactose quiet group increased. (2) The results of IL-6 immunofluorescence staining and Western blotting showed that the expression of IL-6 in D-galactose-quiescent group was significantly higher than that in saline control group (P <0.05); The positive expression of IL-6 in D-galactose group Cell number (8.32 ± 0.43) was significantly lower than that of D-galactose quiescent group (11.54 ± 0.52) (P <0.05), indicating that continuous swimming exercise can reduce the expression of proinflammatory cytokines and inhibit the inflammatory response in aging rats. (3) The results of IL-10 immunofluorescence staining and Western blotting showed that the number of IL-10 positive cells in D-galactose exercise group (11.22 ± 0.53) was significantly higher than that in D-galactose quiescence group (P <0.05) Continuous swimming exercise can up-regulate anti-inflammatory factor expression level. CONCLUSION: Continuous swimming can improve the learning and memory abilities of aged rats, and may delay aging by inhibiting the expression of IL-6, an inflammatory cytokine, and increasing the level of anti-inflammatory cytokine IL-10.