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利用PCR方法从水稻黄单胞细菌(Xanthomonasoryzae)两个致病变种(pv.oryzae和pv.oryzicola)的12个菌株中,扩增出了编码诱导植物过敏反应蛋白激发子Harpin的3类hrfA(hypersensitiveresponsefunctioningfactorA)同源基因hrf1,hrf2和hrf3.同源性分析表明,这些同源基因推测的编码产物均富含甘氨酸,缺乏酪氨酸,在序列的第45位附近的氨基酸都有一个半胱氨酸.HarpinXoo由来源于水稻白叶枯病菌的hrfA基因编码,产物包括两类,一类代表菌株为JxoⅢ,其中GGG-GG基序的重复数为3个,分子量为15.6kD;另一类代表菌株为Pxo112,产物中GGG-GG基序的重复数为4个,分子量为15.9kD,这两个基因分别命名为hrf1和hrf3.HarpinXooc由来源于水稻条斑病细菌的hrfA基因编码,代表菌株为RS105,其中GGG-GG重复数为2个,分子量为15.3kD,水稻黄单胞菌Harpin分子中的一个半胱氨酸残基是其特有的.将这3类基因与已报道的hpa1和xop1基因编码的氨基酸序列进行聚类分析,结果可以将其分为4类,来源于水稻黄单胞菌的HarpinXoo和HarpinXooc被分在相邻的两类中.对3种产物进行比较研究结果,在相同条件下3个代表菌株JxoⅢ,RS105和Pxo112的hrfA基因(hrf1,hrf2和hrf3)表达蛋白的相对浓度分别为:0.389,0.530,0.083mg/mL.hrf1,hrf2和hrf3在大肠杆菌(Bl21)中的表达
Three strains of hrfA encoding harpin, an inducer of plant hypersensitive response protein, were amplified by PCR from 12 strains of two pathogenic strains of Xanthomonas oryzae (pv.oryzae and pv.oryzicola) hypersensitiveresponsefunctioningfactorA) homologous genes hrf1, hrf2 and hrf3 homology analysis showed that these homologous genes deduced coding products are rich in glycine, lack of tyrosine, in the vicinity of the first 45 amino acids have a cysteamine Acid.HarpinXoo is encoded by the hrfA gene derived from the bacterial blight of rice, the products include two types, one representative strain is Jxo Ⅲ, in which the number of GGG-GG motif is three and the molecular weight is 15.6kD; Strain Pxo112, the product of GGG-GG motif repeat number 4, the molecular weight of 15.9kD, the two genes were named hrf1 and hrf3.HarpinXooc derived from the bacterial leaf streak bacteria hrfA gene encoding, representing strains Is RS105, in which the number of GGG-GG repeat is 2 and the molecular weight is 15.3kD, a cysteine residue in the Harpin molecule of X. hyosiae is characteristic.Three kinds of genes were compared with the reported hpa1 and The xop1 gene encodes the amino acid sequence Cluster analysis, the results can be divided into four categories, derived from Xanthomonas Xylophilus HarpinXoo and HarpinXooc were divided into two adjacent categories.For the three kinds of products were compared, the results under the same conditions 3 The relative concentrations of the hrfA genes (hrf1, hrf2 and hrf3) expressing the representative strains JxoIII, RS105 and Pxo112 were 0.389, 0.530 and 0.083 mg / mL respectively, and the expression of hrf1, hrf2 and hrf3 in E. coli (Bl21)