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本文着重讨论钩端螺旋体型特异性脂多糖(简称LPS-F_4)抗血清的制备方法及其杀钩端螺旋体的作用,并进一步研究黄疸出血型钩端螺旋体LPS-F_4抗血清的调理素活性及保护特性,以及用LPS制品给动物免疫后对钩端螺旋体感染的保护能力。一、钩端螺旋体LPS-F_4抗血清的制备与特性:以LPS-F_4(10毫克/毫升)与福氏完全佐剂混合给家兔皮内接种,或以LPS-F_4致敏红细胞作家兔腹腔免疫,并以同型制品的类似剂量给豚鼠、小白鼠及澳洲跳鼠接种。结果发现所有动物在免疫第21～28天,血清中抗体效价达最高峰。如不给追加免
This article focuses on the preparation method of Leptospira-specific lipopolysaccharide (LPS-F_4) antiserum and its role in the detection of Leptospira interrogans and Leptospira interrogans LPS-F_4 antiserum, Protective properties, and the ability to protect Leptospira following immunization of animals with LPS preparations. First, the preparation and characteristics of Leptospira LPS-F_4 antiserum: LPS-F_4 (10 mg / ml) and Freund’s complete adjuvant to rabbits intradermal vaccination, or LPS-F_4 sensitized erythrocytes for rabbit abdominal cavity Immunized and vaccinated guinea pigs, mice and Australian jumping rats at similar doses of the same type of product. The results showed that all animals in the 21st to 28th day of immunization, serum antibody titer reached the peak. If you do not give additional free