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目的 制作阿霉素诱导大白鼠胎仔出现先天性十二指肠闭锁的动物模型 ,并探讨十二指肠闭锁与伴发的胰腺、肝脏发育不良的关系。方法 Wister孕鼠 10只 ,分为实验组 7只 ,对照组 3只 ,分别于妊娠第 6～ 9d腹腔注射阿霉素 1.75mg/kg或等体积的生理盐水 ,妊娠 2 0d剖宫取胎仔 ,对十二指肠、肝脏及胰腺做解剖、电镜及光镜观察。结果 阿霉素组获取胎仔 6 7只 ,十二指肠闭锁 35只 ,占 5 2 .2 % (35 / 6 7) ,其中腔内闭锁 1只 ,占 2 .9% (1/ 35 ) ;十二指肠闭锁近远端由纤维带或胰腺组织充填者 2 7只 ,占 77.1% (2 7/ 35 ) ,闭锁近远端分别为游离盲端者 7只 ,占 2 0 .0 % (7/ 35 )。肝脏畸形 2 2只 ,占 32 .8% (2 2 / 6 7) ,其中 2 1只同时存在十二指肠闭锁畸形。大体形态改变表现为部分肝分叶的缺失 ,尾状叶缺失 14例 ,占 6 3.6 % (14 / 2 2 ) ,5只尾状叶及乳突叶同时缺失 ,占 2 2 .7% (5 / 2 2 ) ,3例尾状叶、乳突叶与肝右叶均缺失 ,占 13.6 % (3/ 2 2 )。镜下观察见部分肝小叶呈发育不良改变。胰腺异常36只 ,占 5 3.7% (36 / 6 7) ,其中 34例同时存在十二指肠闭锁畸形。大体形态改变主要表现在胰腺体积变小 ,2 3只胰颈、体、尾部均缺如 ,占 6 3.9% (2 3/ 36 ) ,13只胰体、尾部缺如 ,占 36 .1% (13/ 36 )。组
Objective To study the animal model of adriamycin-induced congenital duodenal atresia in fetal rats and to explore the relationship between duodenal atresia and associated pancreatic and hepatic dysplasia. Methods 10 Wister pregnant rats were randomly divided into experimental group (n = 7) and control group (n = 3). The rats were injected intraperitoneally with doxorubicin 1.75mg / kg or an equal volume of normal saline on the 6th to 9th day of gestation, The duodenum, liver and pancreas anatomy, electron microscopy and light microscopy. Results There were 67 fetuses and 35 duodenal atresia in the doxorubicin group, accounting for 52.2% (35/67) in the doxorubicin group, 1 of which (1.35%) was blocked in the cavity. Near the distal end of the duodenal atresia by fiber band or pancreas tissue filled with 27 patients, accounting for 77.1% (27/35), at the proximal and distal ends of the blind were free 7, accounting for 20.0% ( 7/35). Twenty-two liver malformations, accounting for 32.8% (2 2/67), of which 21 had concurrent duodenal atresia. The general morphological changes showed the absence of partial hepatic lobes, 14 cases of caudate lobe missing, accounting for 6 3.6% (14/2 2), 5 caudate lobes and mastoid lobes missing, accounting for 22.7% (5) / 2 2), 3 cases of caudate lobe, mastoid lobe and right lobe of liver were missing, accounting for 13.6% (3/2 2). Microscopic observation showed some of the hepatic lobule was dysplastic changes. There were 36 cases of pancreatic abnormalities, accounting for 53.7% (36/67) of them, of which 34 cases had duodenal atresia. The gross morphological changes mainly showed that the size of the pancreas became smaller, the size of the pancreas was smaller than that of the pancreas (23.9%, 23/36) 13/36). group