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对72例月经周期正常的健康妇女,分别按月经周期的不同时间,取子宫内膜,以直接法制备染色体标本。结果表明,①子宫内膜中期分裂相,于月经周期的第5～10d即达高峰,持续到月经周期第16d;于周期第17～22d,分裂相明显减少;至月经第23d以后,内膜细胞的有丝分裂活动几近停止。②在正常月经周期的第14d左右可发生排卵,排卵后内膜初受孕酮影响的时间至少在排卵后36～48h,不会严重干扰中期分裂相的出现。③从子宫前壁或后壁内膜取材直接制备染色体标本进行比较,二者无显著性差异(P>0.05)。④所取组织量与获得中期分裂相数目间呈正相关,有极显著意义(P<0.01)。
In 72 healthy women with normal menstrual cycle, the endometrium was taken at different times of the menstrual cycle, and chromosomal specimens were prepared by direct method. The results showed that: (1) The metaphase of endometrium reached the peak on the 5th to 10th day of the menstrual cycle and continued to the 16th day of the menstrual cycle; on the 17th to 22nd of the cycle, the mitotic phase decreased significantly; after the 23rd day of menstruation, Mitotic activity of cells almost stopped. ② in the normal menstrual cycle 14th or so ovulation can occur, after ovulation intimal primogen progesterone affect the time at least after ovulation 36 ~ 48h, will not seriously interfere with the appearance of metaphase. ③ directly from the anterior wall of the uterus or the posterior wall of the material prepared for comparison, no significant difference between the two (P> 0.05). (4) There was a positive correlation between the amount of tissue taken and the number of metaphase cells (P <0.01).