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目的:制备及表征罗丹明B分子印迹毛细管整体柱并用于样品检测。方法:采用新型三元混合致孔剂,经紫外光引发、原位聚合制备罗丹明B分子印迹固相微萃取毛细管整体柱,用蠕动泵在一定的压力下洗脱模板分子,考察制备及洗脱过程中的主要因素;采用HPLC法对柱性能进行表征。色谱条件:色谱柱为Acclaim 120 C18柱(5μm,4.6 mm×250 mm),流动相为甲醇-水(75∶25),流速0.6 mL·min-1,检测波长550 nm;对市售辣椒样品中的罗丹明B进行测量。结果:探索并确定了该柱的合成、洗脱及应用条件,实际样品测量的回收率介于102%~109%。结论:该柱对罗丹明B具有高灵敏度及选择性,可以对罗丹明B样品进行简单、高效的前处理。
OBJECTIVE: To prepare and characterize Rhodamine B imprinted capillary monolith and use it for sample testing. METHODS: Rhodamine B molecular imprinted solid-phase microextraction capillary monolith was prepared by in-situ polymerization using a novel ternary mixed porogen. The template molecules were eluted with a peristaltic pump under a certain pressure. The effects of preparation and washing The main factors in the process of removal; using HPLC method to characterize the column performance. Chromatographic conditions: Acclaim 120 C18 column (5 μm, 4.6 mm × 250 mm) was used as the mobile phase. The mobile phase was methanol-water (75:25), the flow rate was 0.6 mL · min-1 and the detection wavelength was 550 nm. In rhodamine B measurement. Results: The synthesis, elution and application conditions of the column were explored and confirmed. The recoveries of real samples ranged from 102% to 109%. Conclusion: The column has high sensitivity and selectivity for rhodamine B, which can be used for simple and efficient pretreatment of rhodamine B samples.