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研究目的探讨肝硬化腹水患者与血浆心房利钠尿肽含量的关系。研究设计用放射免疫测定法检测8例肝硬化腹水患者和10例正常人血浆及心房利钠尿肽水平,井进行统计学处理。研究单位生物化学研究室研究对象肝硬化腹水患者8例,男7例,女1例;年龄38～67岁,平均59岁。正常健康人10例;男8例,女2例,平均年龄31岁。处理方法被检查者空腹,抽静脉血3ml,置于含10％EDTA-Na_2 30μl和抑肽酶50μl的玻璃管中混匀。4℃3 000rpm离心10min,取血浆分装两管,-20℃以下保存待测。测定结果放射免疫测定法检测。8例肝硬化腹水患者和10例正常人血浆心房利钠尿肽水平分别为863.7±98.5pg/ml和354.5±39.2pg/ml(P<0.001)。结论肝硬化腹水患者血浆心房利钠尿肽水平明显升高。
Objective To investigate the relationship between plasma atrial natriuretic peptide and cirrhosis patients with ascites. The study designed radioimmunoassay to detect plasma and atrial natriuretic peptide levels in 8 cirrhotic ascites patients and 10 normal controls and to conduct statistical analysis. Research Unit, Department of Biochemistry Laboratory Ascites in patients with cirrhosis in 8 cases, 7 males and 1 female; aged 38 to 67 years, mean 59 years. 10 normal healthy people; 8 males and 2 females, average age 31 years old. Treatment methods were fasting, venous blood pumping 3ml, placed in 10% EDTA-Na_2 30μl and aprotinin 50μl glass tube and mix. Centrifuged at 3 000 rpm at 4 ° C for 10 minutes, aliquoted into two tubes, and stored under -20 ° C until test. The results of radioimmunoassay detection. Plasma atrial natriuretic peptide levels were 863.7 ± 98.5 pg / ml and 354.5 ± 39.2 pg / ml in 8 patients with cirrhosis and 10 normal controls, respectively (P <0.001). Conclusions The level of plasma atrial natriuretic peptide in patients with cirrhosis and ascites is significantly increased.