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目的了解宁夏回族自治区2009年手足口病(Hand,Foodand Mouth Disease,HFMD)流行的病原,探讨病毒分离与逆转录-聚合酶链反应(Reverse Transcription-Polymerase Chain Reaction,RT-PCR)两种方法敏感性的差别。方法采集HFMD患者的疱疹液、粪便、咽拭子标本,进行病毒分离;同时用肠道病毒(Entervirus,EV)通用引物和EV71型(EV71)、柯萨奇病毒A组16型(Coxsakievirus Group A Type16,CA16)特异性引物进行RT-PCR鉴定。结果在采集到标本的344例患者,经检测证实EV71感染的有66例,占总阳性的43.42%;CA1625例,占总阳性的16.45%;其他EV61例,占总阳性的40.13%。病毒分离与RT-PCR两种方法检出率的差异无统计学意义。结论引起宁夏回族自治区2009年HFMD主要病原是EV71。两种方法检测HFMD敏感性一致。
Objective To understand the epidemic pathogens of Hand, Food and Mouth Disease (HFMD) in 2009 in Ningxia Hui Autonomous Region and to explore the relationship between virus isolation and reverse transcription-polymerase chain reaction (RT-PCR) Sexual differences. Methods The herpes fluid, feces and throat swab samples of HFMD patients were collected for virus isolation. At the same time, the common primers of Enterovirus (EV), EV71 (EV71), Coxsakievirus Group A Type16, CA16) specific primers for RT-PCR identification. Results Among the 344 patients collected, 66 cases were EV71 infection, accounting for 43.42% of the total positive cases. CA1625 cases accounted for 16.45% of the total positive cases. Other EV61 cases accounted for 40.13% of the total positive cases. There was no significant difference in detection rate between virus isolation and RT-PCR. Conclusion The main cause of HFMD in 2009 in Ningxia Hui Autonomous Region was EV71. Both methods are consistent in detecting HFMD sensitivity.