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节能减排的生物预处理技术是促进木质纤维素酶水解转化乙醇的有效途径。本试验首次研究了白腐菌杂色云芝(Trametes vesicolor)生物预处理对柳木(Salix babylonica,硬木)和杉木(Cunninghamia lanceolata,软木)纤维素酶水解的影响及作用机制。结果显示生物预处理使硬木和软木的最终转化率分别增加4.78倍和4.02倍。通过研究酶与基质的相互作用发现,预处理后木材基质与酶亲和力的增强并不一定导致酶水解初始转化率的提高;但水解过程中转化速率的下降速度随着解吸附指数增加而降低,说明生物处理主要通过减少纤维素酶对基质的不可逆吸附,延缓水解过程中基质转化速率的急剧下降,从而提高水解效率。不可逆吸附的降低与预处理过程中木质素的部分降解与改性有一定关系。
Biological pretreatment of energy-saving emission reduction technology is to promote lignocellulose hydrolysis and conversion of ethanol an effective way. In this study, the effects of biological pretreatment with Trametes vesicolor on the hydrolysis of cellulase in Salix babylonica (hardwood) and Chinese fir (Cunninghamia lanceolata) were studied for the first time. The results showed that the biological pretreatment increased the final conversion of hardwood and softwood by 4.78 times and 4.02 times respectively. Through the study of the interaction between enzyme and substrate, it was found that the increase of the affinity of wood substrate and enzyme did not necessarily lead to the increase of the initial conversion rate of enzymatic hydrolysis. However, the rate of decline of the conversion rate decreased with the increase of desorption index, Biological treatment mainly through the reduction of cellulase on the substrate irreversible adsorption, delay hydrolysis during the matrix conversion rate dropped sharply, thereby enhancing the efficiency of hydrolysis. The decrease of irreversible adsorption is related to the partial degradation and modification of lignin during the pretreatment.