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目的探讨辛伐他汀对脑缺血再灌注大鼠海马脑源性神经营养因子(brain-derived neurothrophic factor,BDNF)和基质细胞衍生因子-1(stromal cell derived factor-1,SDF-1)的影响。方法 2015年3月—2016年3月选取清洁级大鼠60只,随机分为正常组、治疗组和未治疗组各20只。10%水合氯醛麻醉,暴露并钳夹双侧颈总动脉40 min,造模使大鼠急性脑缺血。治疗组:造模后给予每日灌服辛伐他汀1 mg/kg,未治疗组:造模后胃灌盐水1次/d,正常组:正常饮食。3周后,测量大鼠脑梗死面积,分别测定三组大鼠脑海马CA1区BDNF和SDF-1蛋白的表达情况。计量资料多组间比较采用单因素方差分析,两组间比较采用t检验,P<0.05为差异有统计学意义。结果治疗组的脑梗死面积为(16.24±4.48)%,未治疗组的脑梗死面积为(32.56±6.73)%,两组大鼠的脑梗死面积比较,差异有统计学意义(t=9.028,P<0.05)。三组大鼠BDNF和SDF-1蛋白表达[(83.32±11.46)、(61.25±12.31)、(100.67±10.36)pg/ml与(2.48±0.83)、(0.78±0.96)、(3.23±0.87)ng/μl]比较,差异均有统计学意义(均P<0.05);两两比较发现,正常组BDNF和SDF-1蛋白表达明显高于治疗组和未治疗组,差异均有统计学意义(均P<0.05)。治疗组BDNF和SDF-1蛋白表达明显高于未治疗组,差异均有统计学意义(均P<0.05)。结论辛伐他汀可以提高BDNF和SDF-1蛋白水平,增加内源性保护机制和神经干细胞增殖、迁移,保护神经元的功能。
Objective To investigate the effect of simvastatin on the expression of brain-derived neurothrophic factor (BDNF) and stromal cell derived factor-1 (SDF-1) in rat hippocampus after cerebral ischemia-reperfusion . Methods From March 2015 to March 2016, 60 clean-grade rats were selected and randomly divided into normal group, treatment group and untreated group. Anesthesia was induced by 10% chloral hydrate. The bilateral common carotid arteries were exposed and clamped for 40 minutes. The model rats were subjected to acute cerebral ischemia. Treatment group: given simvastatin 1 mg / kg daily after modeling, untreated group: gastric perfusion saline 1 / d after modeling, normal group: normal diet. After 3 weeks, the area of cerebral infarction in rats was measured and the expression of BDNF and SDF-1 protein in hippocampal CA1 area were measured respectively. Measurement data were compared between groups using one-way ANOVA, t-test was used to compare the two groups, P <0.05 was considered statistically significant. Results The area of cerebral infarction was (16.24 ± 4.48)% in the treatment group and (32.56 ± 6.73)% in the untreated group, and the difference was statistically significant (t = 9.028, P <0.05). The expressions of BDNF and SDF-1 in the three groups were significantly higher than those in the control group (83.32 ± 11.46, 61.25 ± 12.31, 100.67 ± 10.36, and 2.48 ± 0.83, ng / μl], the differences were statistically significant (all P <0.05). Comparing the two groups showed that the expression of BDNF and SDF-1 protein in normal group was significantly higher than that in the untreated and untreated groups (all P <0.05) All P <0.05). The expression of BDNF and SDF-1 protein in the treatment group was significantly higher than that in the untreated group (all P <0.05). Conclusion Simvastatin can increase the levels of BDNF and SDF-1 protein and increase the endogenous protective mechanisms and the proliferation, migration and neuronal protection of neural stem cells.