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本文报导了五名外周血像正常的不同疾病患者的骨髓在半固体琼脂培养中集落生成单位(CFU-c)的剂量活存曲线。3例胸廓成形术病人(2例患纤维空洞肺结核,1例患慢性结核性脓胸)的骨髓取自切除的肋骨;另2例(骨髓无转移的骨肉瘤和Даун氏病各1例)是无菌穿刺取得骨髓。骨髓悬液在融冰温度下以~(60)Co-γ射线照射,剂量率为100拉德/分。应用双层琼脂培养法在含有10%二氧化碳的空气中于37℃培养10～12天。往含有0.5%琼脂的培养基的下层,按浓度为10~6个/毫升,加入供体外周血白细胞,作为集落生成刺激因子(CSF);往含0.3%琼脂的上层按1～2×10~5个/毫升的浓度,加入骨髓细胞。培养结束后在70倍放大镜下计数超过50个细胞的集落,不足50个细胞的则属细胞丛。
This article reports the dose-survival curve of colony-forming units (CFU-c) in bone marrow of five peripheral blood-normal patients with different diseases in semi-solid agar culture. Three cases of thoracic angioplasty patients (two with fibrous hollow tuberculosis and one with chronic tuberculous empyema) were removed from resected ribs; the other two cases (one case of bone marrow non-metastatic osteosarcoma and one case of Даун’s disease) were Aseptic puncture to obtain bone marrow. The bone marrow suspension was irradiated with ~ (60) Co-γ-rays at a melting temperature of 100 rad / min. Double-layer agar culture was used for 10 to 12 days at 37 ° C in air containing 10% carbon dioxide. To the lower layer of medium containing 0.5% agar, donor peripheral blood leukocytes were added as a colony-forming stimulating factor (CSF) at a concentration of 10 to 6 cells / ml; and 1 to 2 × 10 ~ 5 / ml, bone marrow cells were added. After culturing, colonies of more than 50 cells were counted under a 70x magnifying glass, and cell bundles of less than 50 cells.