论文部分内容阅读
Objective:To study expressions of netrin-1 and its receptor UNC5 C in female precocious puberty rat hypothalamus,and explore its effect on precocious puberty process.Methods:Forty female one-week-old SD rats were randomly divided into four groups:experimental group A(precocious puberty early youth),experimental group B(precocious puberty medium youth),group A(normal pre-puberty).group B(normal early youth) with 10 rats in each group.Precocious puberty experimental rats were induced with Danazol and rals in control group were injected with saline.Uterus and ovaries were removed,specimens were weighed,litems index and ovarian index were calculated,and amount of luteinizing hormone(LH) and follicle-stimulating hormone(FSH) were detected from the blood by ELISA.Real-time PCR was used to delect netrin-1 and its receptor UNC5 C.as well as hypothalamic gonadolropinreleasing hormone(GnRH) mRNA expression in hypothalamus tissues:and then,a coimmunoprecipitation study of interactions between netrin-1 and its receptor UNC5 C was carried out.Results:Relative target gene expression levels of control group A,control group B.experimental group A,and experimental group B(with,3-actin as an internal control for normalization) were as follows:Netrin-1:3.5±0.9,5.4±0.7.4.9±1.0.5.3±0.3:UNC5C:0.8±0.04.1.7±0.2.1.82±0.23.1.58±0.4:GnRH:1.2±0.3.2.7±0.3.2.4±0.7.3.2±0.4.Conclusions:LH and FSH concentrations,netrin-1 and its receptor expression are increased in precocious puberty animal models.
Objective: To study expressions of netrin-1 and its receptor UNC5 C in female precocious puberty rat hypothalamus, and explore its effect on precocious puberty process. Methods: Forty female one-week-old SD rats were randomly divided into four groups: experimental group A (precocious puberty early youth), experimental group B (precocious puberty medium youth), group A (normal early-youth) with 10 rats in each group. Precocious puberty experimental rats were induced with Danazol and rals in control group were injected with saline. Uterus and ovaries were removed, specimens were weighed, litems index and ovarian index were calculated, and amount of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) were detected from the blood by ELISA . Real-time PCR was used to delect netrin-1 and its receptor UNC5 C. as well as hypothalamic gonadolropinreleasing hormone (GnRH) mRNA expression in hypothalamus tissues: and then, a coimmunoprecipitation study of interactions between net rin-1 and its receptor UNC5 C was carried out. Results: Relative target gene expression levels of control group A, control group B. experimental group A, and experimental group B (with, 3-actin as an internal control for normalization) were as follows: Netrin-1: 3.5 ± 0.9, 5.4 ± 0.7.4.9 ± 1.0.5.3 ± 0.3: UNC5C: 0.8 ± 0.04.1.7 ± 0.2.1.82 ± 0.23.1.58 ± 0.4: GnRH: 1.2 ± 0.3.2.7 ± 0.3 .2.4 ± 0.7.3.2 ± 0.4. Conclusions: LH and FSH concentrations, netrin-1 and its receptor expression are increased in precocious puberty animal models.