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目的:探讨蔡氏扶正消癥汤对人胃癌细胞SGC-7901体外生长及细胞黏附分子的影响。方法:四甲基偶氮唑蓝(3-[4,5-dimethylthiazol-2-y1]-2,5dipheny1tetrazolium bromide,MTT)还原法检测蔡氏扶正消癥汤对SGC-7901细胞增殖的影响;流式细胞仪检测细胞膜表面E-Cadherin,CD44,CD44V6和CD54的表达。结果:蔡氏扶正消癥生药能抑制SGC-7901的增殖。在实验浓度范围内,随着剂量的增加,抑制作用更加明显;同一剂量下,72h内药物作用时间越长,抑制作用越明显。蔡氏扶正消癥汤作用48h后,SGC-7901细胞膜表面的E-Cadherin表达增高,CD44、CD44V6和CD54表达减少。结论:蔡氏扶正消癥汤能够抑制人胃癌细胞SGC-7901的体外增殖,同时能增加E-Cadherin的表达,减少其CD44,CD44V6和CD54的表达,阻止其对邻近正常组织的浸润及远处转移,从而发挥抗癌作用。
Objective: To investigate the effect of Cai’s Fuzheng Xiaozheng Tang on the growth and cell adhesion molecules of human gastric cancer cell SGC-7901 in vitro. Methods: The effects of Cai’s Fuzheng Xiaozheng Decoction on the proliferation of SGC-7901 cells were detected by MTT reduction method. The effects of flow cytometry Cytometry was used to detect the expression of E-Cadherin, CD44, CD44V6 and CD54 on the cell membrane. Results: Chuaizhengzheng Xiaoji drug can inhibit the proliferation of SGC-7901. In the experimental concentration range, with the increase of dose, the inhibitory effect is more obvious; the same dose, the longer the 72h drug action, the more obvious inhibition. After 48h, the expression of E-Cadherin in SGC-7901 cell surface was increased and the expression of CD44, CD44V6 and CD54 were decreased. Conclusion: Cai’s Fuzheng Xiaozheng Decoction can inhibit the proliferation of human gastric cancer cell SGC-7901 in vitro, increase the expression of E-Cadherin, decrease the expression of CD44, CD44V6 and CD54, and prevent its invasion and distant metastasis to adjacent normal tissues , Which play a role in cancer.