Construction,expression and characterization of tissue-type plasminogen activator mutants

来源 :中国科学：化学英文版 | 被引量 : 0次 | 上传用户：zitayangxin2

【摘要】

：

Three tissue-type plasminogen activator(t-PA)mutants were constructed by recombinant andsite-directed mutagenesis techniques.They are del(296—302)with deletion

【作者】

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刘士辉黄培堂黄翠芬

【机构】

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BeijingInstituteofBiotechnology

【出处】

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中国科学：化学英文版

【发表日期】

：

1995年11期

【关键词】

：

tissue-type PLASMINOGEN ACTIVATOR MUTANT plasma haft-life PAI-1 tissue-type plas

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Three tissue-type plasminogen activator(t-PA)mutants were constructed by recombinant andsite-directed mutagenesis techniques.They are del(296—302)with deletion of PAI-1 binding site,N117Q/N184Qwith deglycosylation of K1 and K2 domains,and their combinatio

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Construction,expression and characterization of tissue-type plasminogen activator mutants

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