论文部分内容阅读
目的 :探讨SP对肿瘤细胞系HL60及L61 5生长的影响。方法 :将SP与两株肿瘤细胞共温育 ,用活细胞计数和MTT试验观察细胞生长趋势 ,并通过电镜观察、流式细胞术分析、G蛋白活性及PKC活性测定等方法探讨该作用的分子机制 ,最后用动物体内实验验证经SP处理后细胞的
Objective: To investigate the effect of SP on the growth of tumor cell lines HL60 and L61 5. METHODS: SP cells were co-incubated with two tumor cells. The viability of the cells was observed by viable cell count and MTT assay. The effect of the molecule was also investigated by electron microscopy, flow cytometry analysis, G-protein activity and PKC activity assay Finally, animal experiments in vivo were used to verify the SP-treated cells