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采用ＭｕｔａｔｉｏｎｓｐｅｃｉｆｉｃＰＣＲ方法，检测１０例ＨＢＶ家族聚集性感染的乙型肝炎病人及其配偶和家族成员ＨＢＶ前Ｃ区１８９６位基因Ｇ→Ａ突变情况，并探讨其临床意义。结果：病人及家族成员突变发生率分别为７０％（７／１０），６６．６％（１４／２１）；明显高于病人配偶１０％（１／１０）。且配偶中乙肝抗ＨＢｓ阳性率为６０％（６／１０）。同时，这种突变在慢性乙型肝炎发生率较高，为８５．７％（６／７），在ＨＢＶ携带者为４４．４％（８／１８），慢性重症肝炎为３３．３％（１／３）。提示这种突变的发生可能与ＨＢＶ持续感染有关。
Mutationspecific PCR method was used to detect the G → A mutation in 1896 HBV pre-C region of 10 hepatitis B patients with HBV familial infection and their spouses and their members, and to explore its clinical significance. Results: The incidence of mutations in patients and their family members were 70% (7/10) and 66.6% (14/21) respectively, which was significantly higher than that of the patients’ spouses (1/10). The positive rate of anti-HBs in spouses was 60% (6/10). At the same time, this mutation was found to be 85.7% (6/7) in chronic hepatitis B, 44.4% (8/18) in HBV carriers and 33.3% in patients with chronic severe hepatitis 1/3). Suggesting that the occurrence of this mutation may be associated with persistent HBV infection.