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目的:观察通络救脑注射液对大鼠局灶性脑缺血再灌注损伤的保护作用及探讨其发挥保护作用的可能途径。方法:以线栓法构建大鼠大脑中动脉局灶缺血再灌注损伤模型,实验动物随机分为假手术组、模型组、通络救脑药物组、阳性药对照(依达拉奉)组。各设3个时间点,分别为脑缺血再灌注后24h、3d和7d,以TTC染色法测定脑组织梗死体积;以酶联免疫(ELISA)、免疫组化法分别检测血清、脑组织匀浆IGF-1表达水平及表达部位。结果:通络救脑注射液能显著减小各时间点的大鼠脑梗死体积比;缺血再灌注3d通络救脑组大鼠血清IGF-1水平显著升高,模型组和通络救脑组大鼠脑匀浆IGF-1水平与假手术组比较显著提高;免疫组化亦提示缺血侧皮层IGF-1的阳性表达面积比在通络救脑组均显著高于相应时间点的模型组。结论:通络救脑注射液能显著减小局灶性脑缺血再灌注大鼠的脑梗死体积,且与提高IGF-1的表达有关。
OBJECTIVE: To observe the protective effect of Tongluo Jiunao injection on focal cerebral ischemia-reperfusion injury in rats and explore the possible ways to play a protective role. METHODS: Rat middle cerebral artery ischemic reperfusion injury model was established by a thread embolism method. Experimental animals were randomly divided into sham operation group, model group, Tongluo Jiunao drug group, and positive drug control group (Edaravone) group. . Each time point was set at 3, 24, 3, and 7 days after cerebral ischemia and reperfusion respectively. Infarct volume was measured by TTC staining; serum and brain tissue were measured by enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry, respectively. Plasma IGF-1 expression levels and expression sites. Results: Tongluo Jiunao Injection can significantly reduce the volume ratio of cerebral infarct at each time point; IGF-1 level in serum of Tongluo Jiunao group was significantly increased after 3 days of ischemia-reperfusion, and the model group and Tongluo save The level of IGF-1 in the brain homogenate of rats was significantly higher than that in the sham group. Immunohistochemistry also suggested that the positive expression area of IGF-1 in the ischemic cortex was significantly higher than that in Tongluo Jiunao group. Model group. Conclusion: Tongluo Jiunao Injection can significantly reduce the cerebral infarction volume in rats with focal cerebral ischemia and reperfusion, and is related to the increase of IGF-1 expression.