应用PCR及寡核苷酸探针研究人原发性肺癌中Ki-ras点突变

• 作 者: 覃扬 孙芝琳 赵建升 孙宁 步宏 刘开凤
• 机 构: 华西医科大学生化教研室,华西医科大学生化教研室,华西医科大学生化教研室,华西医科大学生化教研室,华西医科大学病理教研室,华西医科大学病理教研室 成都 610041,成都 610041,成都 61004
• 出 处: 生物化学与生物物理进展
• 发表日期: 1992年04期
• 关键词: 原发性肺癌 Ki-ras PCR 点突变 寡核苷酸探针 小细胞肺癌 石蜡切片标本 人肺癌 突变率 分化程度

应用PCR及寡核苷酸探针杂交方法研究了50例人原发性肺癌石蜡切片标本 中Ki-ras 12(Val),Ki-ras 13(Cys)点突变,发现13例为点突变阳性,突变率为26％,其中3例同时兼有二种点突变(Val/Cys);Ki-ras 12位的点突变率(20％)比Ki-ras 13位的点突变率(12％)高。同时还发现Ki-ras基因的点突变存在于腺癌、鳞癌、小细胞肺癌以及各种分化程度的肺癌中。

The mutations of Ki-ras 12 (Val) and Ki-ras 13 (Cys) in 50 human primary lung cancer paraffin sections were studied by PCR and oligonucleotide probe hybridization. 13 of them were found to be point mutation-positive, The mutation rate was 26%. Three of them also had two kinds of point mutations (Val / Cys). The point mutation rate (12%) in Ki-ras at position 12 was higher than that at point 13 in Ki-ras . Point mutations of Ki-ras gene were also found in adenocarcinoma, squamous cell carcinoma, small cell lung cancer and various degrees of differentiation of lung cancer.