论文部分内容阅读
目的 研究已证实维甲酸受体 -β( Retinoic acid receptor-beta,RARβ)对某些类型的恶性肿瘤细胞有诱导分化和凋亡作用。现观察转导 RARβ基因联合全反式维甲酸 ( all trans retinoic acid,ATRA)对 Tca8113细胞的凋亡作用。方法 先应用脂质体将 RARβ基因导入 Tca8113细胞中 ,然后采用活细胞计数、透射电镜、流式细胞仪等方法观察 ATRA对转基因细胞的凋亡影响和生长抑制作用。结果 转 RARβ基因 Tca8113细胞在 1μmol/LATRA作用 10天后 ,细胞生长抑制率达 90 .2 % ;光镜和透射电镜下观察都可见到典型形态变化的凋亡细胞 ;FCM测定结果表明 ,细胞凋亡率高达 80 .7%。而母本细胞 Tca8113凋亡率小于 2 0 %。结论 RARβ基因可能是 ATRA诱导 Tca8113细胞产生显著凋亡作用的“靶分子”。 RARβ基因联合维甲酸治疗某些口腔鳞癌将是有效和可行的。
Objective To study the effect of Retinoic acid receptor-beta (RARβ) on differentiation and apoptosis of some types of malignant tumor cells. The effect of transduction of RARβ gene combined with all trans retinoic acid (ATRA) on Tca8113 cells was observed. Methods Liposomes were used to introduce RARβ gene into Tca8113 cells. The viability and growth inhibition of ATRA on Tca8113 cells were observed by using live cell counting, transmission electron microscopy and flow cytometry. Results Tca8113 cells transfected with TAR8113 cells showed 90.2% growth inhibition after treated with 1μmol / L ATRA for 10 days. Apoptotic cells with typical morphological changes were observed under light and transmission electron microscopy. FCM results showed that apoptosis Rate up to 80.7%. While the rate of apoptosis in the female Tca8113 cells was less than 20%. Conclusion The RARβ gene may be the “target molecule” of ATRA-induced apoptosis in Tca8113 cells. RARβ gene combined with tretinoin in the treatment of some oral squamous cell carcinoma will be effective and feasible.