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通过皮下注射的方法诱导豚鼠产生金属硫蛋白（ＭＴ），研究了重金属元素（Ｃｄ）、微量元素（Ｃｕ，Ｚｎ）及有机试剂（ＣＣｌ４，在体内可产生自由基）等因素的诱导与豚鼠肝脏中ＭＴ不同亚型的含量及金属结合状态的变化关系．实验结果表明，微量元素及有机试剂的诱导可使豚鼠肝脏中ＭＴ１的产量明显高于ＭＴ２，说明在体内ＭＴ１在参与微量元素的储存及清除自由基功能方面比ＭＴ２强．在重金属元素诱导下体内ＭＴ１对重金属元素的结合量远远大于ＭＴ２．表明ＭＴ１的重金属解毒能力比ＭＴ２强．上述实验结果与对不同亚型ＭＴ生物学功能差异的体外研究结果相吻合．此外，无论采用上述何种因素诱导，所得ＭＴ中均结合有Ｃｕ．对Ｃｕ在ＭＴ形成过程中的作用也进行了初步探讨．
The production of metallothionein (MT) was induced by subcutaneous injection in guinea pigs. The effects of heavy metal elements (Cd), trace elements (Cu, Zn) and organic reagents (CCl4, free radicals in the body) The content of different subtypes of MT and the relationship between the metal binding status. The results showed that the induction of trace elements and organic reagents in guinea pig liver MT1 production was significantly higher than that of MT2, indicating that in vivo MT1 involved in the storage of trace elements and scavenger free radical function than MT2. The amount of heavy metal elements bound by MT1 in vivo is much greater than that of MT2 induced by heavy metal elements. MT1 showed that the detoxification of heavy metals stronger than MT2. The above experimental results coincide with the results of in vitro studies on the differences in the biological functions of MT in different subtypes. In addition, whatever the above factors were induced, Cu was incorporated into the resulting MT. The role of Cu in the formation of MT was also discussed.