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目的研究饮用水有机提取物对正常人肝(HL-7702)细胞所致的毒性作用。方法采集某水厂水源水和管网末梢水水样,富集并提取有机物。调整HL-7702细胞悬液密度为2×105/孔,分别加入终浓度为0(对照,二甲基亚砜)、1.25、5、20 ml原水有机提取物/ml,每24 h更换一次培养液,培养72 h。测定细胞的存活率、在各细胞周期的构成比及凋亡率。结果与对照组比较,管网末梢水和水源水各剂量有机提取物染毒HL-7702细胞的存活率均较低,凋亡率较高,差异有统计学意义(P<0.05,P<0.01)。与对照组比较,5、20 ml/ml管网末梢水有机提取物染毒HL-7702细胞G0-G1期细胞构成比较低,S期较高,20 ml/ml有机提取物染毒HL-7702细胞G2-M期细胞构成比较低,差异均有统计学意义(P<0.05)。与对照组比较,5、20 ml/ml水源水有机提取物染毒HL-7702细胞S期细胞构成比较高,20 ml/ml有机提取物染毒HL-7702细胞G2-M期细胞构成比较低,差异均有统计学意义(P<0.05);各剂量有机提取物染毒HL-7702细胞G0-G1细胞构成比无明显变化(P>0.05)。管网末梢水及水源水有机提取物均可导致HL-7702细胞凋亡,并随染毒剂量的增加凋亡率逐渐增加(P<0.05),20 ml/ml组末梢管网水的凋亡率明显高于水源水(P<0.05)。结论在本实验条件下,饮用水有机提取物对HL-7702细胞具有毒性作用,且消毒后的管网末梢水有机提取物的细胞毒性作用更为明显。
Objective To study the toxic effects of organic extracts of drinking water on normal human liver (HL-7702) cells. Methods Water samples from a water plant and the water from the pipe network were collected, and the organic compounds were enriched and extracted. The density of HL-7702 cell suspension was adjusted to 2 × 10 5 / well, and the final concentration of 0 (control, dimethylsulfoxide), 1.25, 5,20 ml of raw organic extract / ml were added every 24 h Liquid, cultured for 72 h. The cell viability, the constituent ratio at each cell cycle and the apoptosis rate were determined. Results Compared with the control group, the survival rate of HL-7702 cells treated with different concentrations of organic extracts of the tube and water sources was lower and the apoptosis rate was higher (P <0.05, P <0.01) ). Compared with the control group, the cells in the G0-G1 phase of HL-7702 cells treated with 5,20 ml / ml of the aqueous extract of the pipe network were relatively low and had a high S phase. The organic extract of 20 ml / ml HL-7702 Cell G2-M phase cells were relatively low, the difference was statistically significant (P <0.05). Compared with the control group, cells in 5-20 ml / ml water-sourced organic extracts of HL-7702 cells at S phase had a higher percentage of cell structure, and cells in 20 ml / ml organic extract at G2-M phase of HL-7702 cells were relatively low (P <0.05). There was no significant difference in the constituent ratio of G0-G1 cells in HL-7702 cells treated with different concentrations of organic extracts (P> 0.05). Apoptosis of HL-7702 cells could be induced by pipe network water and organic extracts of water source, and apoptosis rate increased gradually with the increase of exposure dose (P <0.05). Apoptosis The rate was significantly higher than that of water (P <0.05). Conclusions The organic extract of drinking water has toxic effect on HL-7702 cells under the experimental conditions, and the cytotoxic effect of the organic extract of the peripheral of the pipe network after disinfection is more obvious.