Transplantation of X-box-binding protein-1 gene-modified neural stem cells in the lateral ventricle

• 作 者: Yao Wang Xiaokun Gang Qun Liu
• 机 构: Department of Neurology,Department of Endocrinology
• 出 处: 中国神经再生研究（英文版）
• 发表日期: 2004年期
• 关键词: X-box-binding protein-1 neural stem cells transplantation brain ischemia brain i

X-box-binding protein-1 (XBP-1) is an essential transcription factor in endoplasmic reticulum stress.In this study,XBP-1 gene-transfected neural stem cells (NSCs) were transplanted into lesion sites to ensure stability and persistent expression of XBP-1,resulting in the exertion of anti-apoptotic effects.Simultaneously,XBP-1 gene transfection promotes the survival and differentiation of transplanted NSCs.Results from this study demonstrated that survival,proliferation and differentiation of XBP-1 gene-modified NSCs were enhanced when compared to unmodified NSCs at 28 days post-transplantation (P ＜ 0.05).A diminished number of apoptotic neural cells increased Bcl-2 expression and reduced Bax expression,and were observed in the ischemic region of the XBP-1-NSCs group (P ＜ 0.05).These results indicated that modification of the XBP-1 gene enhances the survival and migration of NSCs in vivo and decreases the occurrence of apoptosis.