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目的:建立HPLC测定安神胶囊中芒果苷的含量测定方法。方法:采用HPLC测定芒果苷的含量,Diamonsil C18(4.6 mm×250 mm,5μm)为色谱柱,流动相乙腈-0.2%冰醋酸水溶液(15∶85),流速1 mL.min-1,检测波长258 nm,进样量10μL。结果:芒果苷在2.5～7.5 mg.L-1呈良好的线性关系(r=0.999 8),回收率99.58%(RSD 0.79%,n=9)。结论:方法简便,准确,灵敏度高,重复性好,可用于安神胶囊中知母有效成分芒果苷的含量测定。
Objective: To establish a method for the determination of mangiferin in Anshen capsules by HPLC. Methods: The content of mangiferin was determined by HPLC. Diamonsil C18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase was acetonitrile-0.2% glacial acetic acid (15:85), the flow rate was 1 mL.min-1. 258 nm, injection volume 10μL. Results: Mangiferin had a good linear relationship (r = 0.999 8) with a recovery of 99.58% (RSD 0.79%, n = 9) at 2.5-7.5 mg · L -1. Conclusion: The method is simple, accurate, sensitive and reproducible. It can be used to determine the content of mangiferin in An Shen Capsule.