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杜仲茎尖接种在上约20天,基部膨大,产生白色絮状愈伤组织,出愈率高达100％,转至中,10—20天后愈伤组织渐渐变成浅黄色或翠绿色,再转至中,在愈伤组织上发生3—7个不定芽,其分化频率高达47.1％。同时杜仲茎尖接种在上,20天左右茎尖生长1.5—3cm,叶片伸展,腋芽萌发伸长成为侧枝,最长侧枝可达2cm,切取腋芽继代培养可以增殖,同主苗一起转入生根培养基诱导生根。对以上两条途径所得无根苗进行不同的生根处理,结果表明:以培养基诱导效果最好,同时健壮且木质化程度稍高的无根苗有利于发根,发根率高达84.1％。另外,分步诱导有利于壮苗。
Eucommia shoot tip inoculation on about 20 days, the base of the enlargement, resulting in white flocculent callus, the rate of up to 100%, to medium, 10-20 days after the calluses gradually become pale yellow or green, and then turn To, 3 to 7 adventitious buds occurred on callus, with a frequency of up to 47.1%. At the same time the euchromataceous shoots inoculated on the 20 days or so shoot tip growth of 1.5-3cm, leaf stretch, axillary buds elongation to become the side branches, the longest side branches up to 2cm, axillary buds cut the subculture can proliferate, with the main seedling into rooting Medium induces rooting. Roots of the above two ways were rooted different treatment, the results showed that: the best induction medium, at the same time strong and more lignified degree of rootless seedling is conducive to the root, hair rooting rate as high as 84.1%. In addition, step-by-step induction is conducive to strong seedlings.