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以滇Ⅰ型杂交粳稻高产组合榆杂 2 9保持系滇榆 1号B、恢复系南 2 9- 1和寻杂 36保持系滇寻 1号B、恢复系南 2 9- 2为材料。采用混合筛选法 ,从 2 0 0个RAPD引物中 ,筛出在两个组合保持系与恢复系间表现RAPD差异的 3个标记 :OPE - 1170 0 ,OPE - 1182 0 ,OPE - 11154 3 。分别随机提取 4个材料的 15 0个单株DNA ,构建 4个随机单株DNA群体。利用OPE - 1170 0 ,OPE - 1182 0 ,OPE - 11154 3 3个标记鉴别随机单株DNA群体。结果表明 :OPE- 1170 0 ,OPE - 1182 0 ,OPE - 11154 33个标记在 4个材料间均能扩增出明显的多态性产物 ,重复性好 ,4个材料的单株正确鉴别率分别为 :滇榆 1号B :97 3 % ,南 2 9- 1:96 % ,滇寻 1号B :95 3 % ,南 2 9- 2 :92 %。
The high-yielding combinations of Yunnan-type japonica hybrid rice Yusan 29 with Dian Yu 1 B, restorer Nan 2 9-1 and Xunza 36 with Dian Xun 1 B and restorer Nan 2 9-2 were selected as materials. Using the hybrid screening method, we screened 200 markers: OPE - 1170 0, OPE - 1182 0 and OPE - 11154 3, which showed the difference in RAPD between the two maintainer and restorer lines from 200 RAPD primers. Fifty individual plant DNAs of four materials were randomly collected and four randomized single DNA populations were constructed. The random DNA strands were identified using 3 markers, OPE - 1170 0, OPE - 1182 0 and OPE - 11154. The results showed that 33 markers of OPE-1170 0, OPE-1182 0 and OPE-11154 could amplify the obvious polymorphism products among the four materials with good reproducibility. The correct identification rates of the four materials were respectively As follows: Yunnan Yu 1 B: 97 3%, South 29- 1:96%, Dian Xun 1 B: 95 3%, South 29-92: 92%.