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用超声波法提取苜蓿皂苷,通过L9(34)正交试验,探讨了提取条件对苜蓿总皂苷提取量的影响。结果显示:各试验因素对苜蓿总皂苷类物质提取量的影响依次为超声时间、物料比、超声功率、超声温度;最佳工艺条件为A2B3C2D1,即物料比1:20、超声时间30min、超声功率80W、超声温度20℃。在上述最佳条件下,总皂苷的提取率可达1.975%。
The alfalfa saponin was extracted by ultrasonic method. The effects of extraction conditions on the total saponin extraction of alfalfa were studied by L9 (34) orthogonal test. The results showed that the influence of each experimental factors on the total saponins extracted from alfalfa were ultrasonic time, material ratio, ultrasonic power and ultrasonic temperature. The optimum technological conditions were A2B3C2D1, material ratio 1:20, ultrasonic time 30min, ultrasonic power 80W, ultrasonic temperature 20 ℃. Under the above optimal conditions, the total saponin extraction rate of up to 1.975%.