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目的探讨新疆哈萨克族食管鳞癌组织中细胞周期蛋白(cyclins)D1 mRNA的表达及其临床意义。方法以cyclin D1 mRNA为靶基因,运用逆转录聚合酶链反应(RT-PCR)法检测48例哈萨克族食管鳞癌患者中cyclin D1mRNA的表达。结果 48例哈萨克族食管鳞癌患者癌组织、手术切缘无癌组织中cyclin D1mRNA表达阳性率为95.75%(45/48)和87.5%(42/48),差异无统计学意义(P>0.05);cyclin D1 mRNA的表达与分化程度相关,与不同浸润深度及有无淋巴结转移均无相关性。结论 cyclin D1 mRNA表达与哈萨克族食管癌的发生、发展、远处转移和细胞分化程度均无密切相关性,其对哈萨克族食管癌发生发展过程中的具体作用有待于进一步研究。
Objective To investigate the expression of cyclin D1 mRNA in Kazakh esophageal squamous cell carcinoma and its clinical significance. Methods cyclin D1 mRNA was used as a target gene and cyclin D1 mRNA expression was detected in 48 Kazakh esophageal squamous cell carcinoma patients by reverse transcription-polymerase chain reaction (RT-PCR). Results The positive rates of cyclin D1 mRNA expression in 48 cases of esophageal squamous cell carcinoma of Kazakh patients were 95.75% (45/48) and 87.5% (42/48) respectively, with no significant difference (P> 0.05 ). The expression of cyclin D1 mRNA was correlated with the degree of differentiation and had no correlation with different depth of invasion and lymph node metastasis. Conclusion The expression of cyclin D1 mRNA is not closely related to the occurrence, development, distant metastasis and cell differentiation of Kazakh esophageal cancer. The specific role of cyclin D1 mRNA in the development and progression of Kazakh esophageal cancer remains to be further studied.