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稻瘟菌致病力易发生变异,过去和现在都有过报道。为了能进行品种遗传研究,日本曾选择了代表不同小种的致病力较为稳定的分离菌。我们也想从现有保存的分离菌中选择致病力较稳定的分离菌,以供抗性遗传研究之用。材料和方法1、供试分离菌和孢子培养从历年测定小种的单孢菌中,选择0137—2(属小种ZA_1)、75—49(属小种ZA_(25))、73—15(属小种ZB_1)、75—72(属小种ZB_(25))02061(属小种ZC_(13))和0035—2(属小种ZG_1)。将大麦粒上保存的原菌株,移于酵母淀粉培养基上培养孢子,用稀释法分离单孢,移至斜面培养基上,长满整个斜面后,
Pathogenicity of Magnaporthe griseus prone to mutation, past and present have been reported. In order to carry out genetic research on cultivars, Japan has selected isolates that represent more stable virulence strains of different races. We also want to select the more pathogenic isolates from existing preserved isolates for resistance genetic research purposes. Materials and methods 1, the test for isolates and spore culture from the past years to determine the species of microsporum, select 0137-2 (a race ZA_1), 75-49 (race ZA_ (25)), 73-15 (Race ZB_1), race 75-72 (race ZB_ (25)) 02061 (race ZC_ (13)) and 0035-2 (race ZG_1). The original strains stored on barley grain, moved to yeast starch culture medium spores, separated by dilution single spore, transferred to the slant medium, covered with the entire slant,