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目的 比较口腔鳞癌肿瘤浸润淋巴细胞 (tum or infiltrating lym phocyte,TIL)和肿瘤引流淋巴结细胞(tum or draining lym ph node lymphocyte,TDL NL)体外杀伤活性。方法 从 5例口腔癌原发灶及其转移灶中分离TIL和 TDL NL ,用 3H - Td R4h释放法对 TDL NL和 TIL对自体肿瘤的杀伤活性进行比较研究。结果 3周时 TIL对自体肿瘤的杀伤活性较 TDL NL强 (P<0 .0 2 ) ,分别为 35 %和 2 3% ;4周时 TDL NL对自体肿瘤的杀伤活性大于TIL (P<0 .0 2 ) ,分别为 5 3%和 40 % ;5周时 TDL NL对自体肿瘤的杀伤活性仍显著强于 TIL (P<0 .0 2 ) ,分别为6 2 %和 44 %。结论 在口腔鳞癌过继免疫治疗中 ,TDL NL可能较 TIL具有更大的优势
Objective To compare the in vitro killing activity of tumor-infiltrating lymphocyte (TIL) and tumor-draining lymphocyte (TDL NL) in oral squamous cell carcinoma. Methods TIL and TDL NL were isolated from 5 cases of primary cancer of the oral cavity and their metastases. The cytotoxicity of TDL NL and TIL to autologous tumor was compared with 3H - Td R4h release assay. Results The cytotoxicity of TIL to autologous tumor at 3 weeks was significantly higher than that of TDL NL (35% vs 23%, respectively). Toxicity of TDL NL to autologous tumor at 4 weeks was greater than that of TIL (P <0) .0 2), respectively, at 5 3% and 40% respectively. Toxicity of TDL NL to autologous tumor at 5 weeks was still significantly higher than that of TIL (P <0. 02), which was 62% and 44% respectively. Conclusion In adoptive immunotherapy of oral squamous cell carcinoma, TDL NL may have more advantages than TIL