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This paper deals with the influences of pH,acidity and ionic intensity of the solutions on the resonance Rayleigh scattering spectra and fluorescence spectra of humic acid.When the pH value is low and the acidity and ionic intensity are high,the resonance Rayleigh spectra and fluorescence spectra both show a tendency of increasing,though the formers intensity is much higher.In combination with the transmission electron microscope data,the factors leading to the occurrence and enhancement of the resonance Rayleigh scattering spectra of humic acid were explored.It is considered that particle enlargement caused by aggregation,the increase of heterogeneity,the increase of hydrophobility,the formation of interface,etc.,are the factors leading to the occurrence and enhancement of the resonance Rayleigh scattering spectra of humic acid.As the intensity of the resonance Rayleigh scattering spectra of humic acid is much higher,resonance Rayleigh scattering spectroscopy can be used as a newly developed spectrum technology,which is more sensitive and simpler,to study humic acid and its complicated behaviors.