论文部分内容阅读
目的探讨直接免疫荧光法(DFA)和多重逆转录聚合酶链反应(RT-PCR)对呼吸道常见病毒人鼻病毒(HRV)感染患者分泌物的检测,判断其临床诊断价值。方法选取2010年2月-2011年12月收集的急性呼吸道感染患儿鼻咽分泌物210例,分别用DFA和RT-PCR进行检测,对两组测定方法的检测结果进行判定。结果多重RT-PCR检出112份阳性标本,阳性率为53.33%,DFA检测出89份阳性标本,阳性率为42.38%,两组比较,差异有统计学意义(χ2=27.623,P<0.01)。结论 DFA检测方法可以广泛适用于临床检测中;多重RT-PCR检测方法的敏感性高,检测病毒的范围广,可以做亚型鉴定方法,适用于呼吸道病毒流行病学的调查研究。
Objective To investigate the detection of secretion of common respiratory virus human respiratory virus (HRV) infection by direct immunofluorescence (DFA) and multiplex reverse transcriptase-polymerase chain reaction (RT-PCR) and to evaluate its clinical value. Methods 210 cases of nasopharyngeal secretions collected from February 2010 to December 2011 in children with acute respiratory tract infection were selected and detected by DFA and RT-PCR, respectively. The results of two test methods were determined. Results The positive rate of 112 positive samples was 53.33% by multiplex RT-PCR, and 89 positive samples were detected by DFA. The positive rate was 42.38%. There was significant difference between the two groups (χ2 = 27.623, P <0.01) . Conclusion DFA detection method can be widely used in clinical detection; Multiplex RT-PCR detection method has high sensitivity, wide range of detection of virus, can be sub-type identification method, suitable for respiratory virus epidemiology research.