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对体外传代培养的人血管起源平滑肌肉瘤细胞进行直接和脂质体Ｔｆｘ－５０包裹的人心钠素（ＡＮＦ）基因表达质粒ｐｃＤＡＮ３／ＡＮＦ转染，并以非同位素地高辛标记的ＲＮＡ分子探针，对转染细胞中的ＡＮＦｍＲＮＡ水平进行ＲＮＡＤｏｔＢｌｏｔ分子杂交检测；同时，利用...
In vitro cultured human angiogenic leiomyosarcoma cells were transfected directly with liposomal Tfx-50 human atrial natriuretic protein (ANF) gene expression plasmid pcDAN3/ANF and probed with a non-isotopic digoxigenin-labeled RNA molecule. , RNADotBlot molecular hybridization detection of ANF mRNA levels in transfected cells; at the same time, using...