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目的观察培养的大鼠皮层神经元损伤后ｃ－ｆｏｓ蛋白表达，探讨其在继发性脑损伤中的作用。方法混合培养１０～１２天ＳＤ胎鼠的皮层神经元分别用谷氨酸和机械划痕损伤。采用ＳＰ法进行免疫组化双重标记反应。结果谷氨酸作用后２小时，ｃ－ｆｏｓ蛋白开始表达，４小时后在核中明显浓聚。创伤后２小时仅在创道边缘直接损伤部位有ｃ－ｆｏｓ蛋白表达，远离创道未直接损伤区的神经元在创伤后４小时才出现；少数神经元突起远端有明显ｃ－ｆｏｓ蛋白颗粒；损伤后６小时神经元突起开始崩解，但ｃ－ｆｏｓ蛋白颗粒仍未消失。结论谷氨酸损伤可引起培养的神经元ｃ－ｆｏｓ蛋白的大量表达，损伤后培养皮层神经元和胶质细胞可以释放出神经毒性物质，培养神经元损伤后ｃ－ｆｏｓ蛋白过度表达可能与凋亡有关
Objective To observe the expression of c-fos protein in rat cortical neurons after injury and to explore its role in secondary brain injury. Methods Cultured cortical neurons of 10 ~ 12 days SD fetal rats were injured with glutamate and mechanical scratches respectively. SP immunohistochemical double labeling reaction. Results Two hours after the glutamate treatment, c-fos protein started to express and in the nucleus was obviously concentrated after 4 hours. The expression of c-fos protein was detected only at the site of direct injury on the edge of the traumatic tract 2 hours after trauma. The neurons far away from the direct lesion site of traumatic tract did not appear until 4 hours after trauma. The number of c-fos protein particles Neuronal processes began to disintegrate 6 hours after injury, but c-fos protein particles did not disappear. Conclusion Glutamic acid can induce the expression of c-fos protein in cultured neurons. Neurons and glial cells can release neurotoxic substances after injury. The overexpression of c-fos protein may play an important role in neuronal injury. Death related