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目的以附子-干姜药对为研究对象,采用高效液相色谱法测定附子单煎液及附子与干姜合煎液中次乌头碱(HA)、苯甲酰乌头原碱(BAC)、苯甲酰次乌头原碱(BHA)及苯甲酰新乌头原碱(BMA)4种乌头类生物碱,探讨两药相须配伍增效原理。方法 Kromasil C18柱(4.6 mm×250 mm,5μm);流动相:A相为乙腈,B相为0.2%醋酸水溶液(三乙胺调pH为6.2),梯度洗脱(0～15 min:25%～31%A;15～36 min:31%～35%A;36～40 min:35%～25%A);流速1.0 mL·min-1,检测波长240 nm,柱温30℃。结果 4种乌头类生物碱均达到基线分离,线性关系良好(r>0.999),加样回收率为96.9%～101.9%,RSD<3%。与附子单煎液相比,附子与干姜合煎液中次乌头碱及苯甲酰新乌头原碱煎出量增加,苯甲酰乌头原碱及苯甲酰次乌头原碱煎出量稍有减少,但4种乌头类生物碱煎出量总和呈增加趋势。结论附子与干姜配伍可促进乌头类总生物碱的溶出,从成分角度解释附子与干姜相须配伍增效原理。
OBJECTIVE To study the effects of aconite-ginger herb on the expression of aconitine (HA), benzoyl aconitine (BAC) , Benzoyl aconitine (BHA) and benzoyl aconitine (BMA) four kinds of aconite alkaloids, to explore the principle of compatibility of two drugs to increase the principle. (4.6 mm × 250 mm, 5 μm). The mobile phase consisted of acetonitrile (acetonitrile), 0.2% acetic acid in phase B (adjusted to pH 6.2 with triethylamine) and gradient elution ~ 31% A; 15 ~ 36 min: 31% ~ 35% A; 36 ~ 40 min: 35% ~ 25% A). The flow rate was 1.0 mL · min-1. Results The four alkaloids from Aconitum reached baseline separation with a good linearity (r> 0.999). The recoveries were 96.9% -101.9% with RSD <3%. Compared with the single-decoction of aconite, the aconitum and decoction of aconitine and aconitine aconitum in decoction of Radix Aconiti Preparata and Dried Ginger decoction increased, the amount of benzoyl aconitine and benzoyl aconitine However, the total amount of 4 aconite alkaloids showed an increasing trend. Conclusion The combination of aconite and ginger can promote the dissolution of total alkaloids of aconitum and explain the principle of compatibility and compatibility between aconite and ginger.