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通过无公害化学诱导子的诱导可使茶树芽叶的EGCG含量提高20.15%～25.00%。为了探明诱导的分子机制,用固相pH梯度双向凝胶电泳分离诱导芽叶与正常芽叶的总蛋白质,结果获得了分辨率和重复性较好的双向电泳图谱,差异表达分析发现,诱导出现的特异蛋白有14种,诱导消失的特异蛋白有8种,诱导表达上调相差10倍的特异蛋白有11种,诱导表达下调相差10倍的特异蛋白有6种。选取两个差异蛋白质点进行基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)测定其酶解后的肽质指纹图谱,通过http:/www.matrixscience.com网站,利用Mascot软件检索NCBInr数据库。查询结果:一种为光合系统Ⅰ的铁硫蛋白,另一种为未知蛋白。这些结果表明,茶树诱导芽叶与正常芽叶的蛋白质组存在差异,这些特异蛋白可能在诱导过程中起着重要的作用。
The EGCG content of tea shoots increased by 20.15% -25.00% through the induction of pollution-free chemical elicitors. In order to explore the molecular mechanism of induction, two-dimensional gel electrophoresis was used to separate the total protein from the bud and the normal shoot using the immobilized pH gradient two-dimensional gel electrophoresis. The results showed that the resolution and repeatability of the two- There are 14 kinds of specific proteins, 8 kinds of specific proteins that induce disappearance, 11 kinds of specific proteins that induce 10 times up-regulation, and 6 kinds of special proteins to induce down-regulation by 10 times. Two differential protein spots were selected for determination of the peptidyl fingerprinting after enzyme digestion by matrix-assisted laser desorption / ionization time-of-flight mass spectrometry (MALDI-TOF-MS), using the Mascot software to search the NCBInr database at http: //www.matrixscience.com . The results are as follows: one is the iron-sulfur protein of photosystem Ⅰ, the other is unknown protein. These results indicate that there are differences in the proteome between bud-induced leaves and normal shoots, and these specific proteins may play an important role in the induction process.