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目的观察基质金属蛋白酶-7(matrix metalloproteinase-7,MMP-7)和基质金属蛋白酶抑制因子-1(tissueinhibitors of matrix metalloproteinase-1,TIMP-1)在子宫内膜异位症(Endmetriosis,EMs)中的表达,探讨二者在EMs发病机制中的作用。方法采用免疫组化链霉菌抗生物素蛋白-过氧化物酶连接(SP)法检测35例EMs患者的在位内膜,异位内膜及20例对照组为因肌壁间或浆膜下子宫肌瘤在我科行子宫切除术,且月经周期正常的子宫内膜中MMP-7和TIMP-1表达情况,检测结果采用SPSS软件进行统计学分析。结果MMP-7在EMs在位内膜中的阳性表达率为57.1%,异位内膜中的阳性表达率45.7%,与对照组子宫内膜组织的表达(阳性率为40.0%)相比无显著性差异(P>0.05)。研究组异位内膜中的表达强度明显高于对照组,两者比较,差异有统计学意义(P<0.05)。TIMP-1在两组所有标本中均有阳性表达。TIMP-1在研究组异位内膜中的表达强度低于在位内膜,两者比较,差异有统计学意义(P<0.05)。研究组在位内膜中的表达强度稍低于对照组,两组比较,差异无统计学意义(P>0.05)。结论MMP-7在EMs的发生过程中起了重要作用。MMP-7在位内膜中表达增强,异位内膜TIMP-1表达减弱,是EMs患者在位内膜、异位内膜细胞具有侵袭能力的原因之一。
Objective To investigate the expression of matrix metalloproteinase-7 (MMP-7) and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in endometriosis (EMs) In order to explore the role of the two in the pathogenesis of EMs. Methods Immunohistochemical streptavidin-peroxidase (SP) method was used to detect the eutopic and ectopic endometrium of 35 patients with EMs and the control group of 20 patients with intrauterine or subserosal uterus Fibroids in our hysterectomy, and the normal menstrual cycle endometrial MMP-7 and TIMP-1 expression, test results using SPSS software for statistical analysis. Results The positive expression rate of MMP-7 in eutopic endometrium of EMs was 57.1%, the positive expression rate of ectopic endometrium was 45.7%, compared with the expression of endometrial tissue in control group (positive rate was 40.0%) Significant difference (P> 0.05). The expression intensity of ectopic endometrium of the study group was significantly higher than that of the control group, the difference was statistically significant (P <0.05). TIMP-1 was positive for all specimens in both groups. The expression intensity of TIMP-1 in the ectopic endometrium of the study group was lower than that in the eutopic endometrium, the difference was statistically significant (P <0.05). The expression intensity of eutopic endometrium of the study group was slightly lower than that of the control group. There was no significant difference between the two groups (P> 0.05). Conclusion MMP-7 plays an important role in the pathogenesis of EMs. The expression of MMP-7 in eutopic endometrium is enhanced, and the expression of TIMP-1 in ectopic endometrium is weakened, which is one of the reasons why eutopic and ectopic endometrial cells have invasive ability in EMs.