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通过连续两年对温敏雄不育小麦Ｃ４９Ｓ的分期播种试验发现，早播（低温）的ⅠＣ４９Ｓ（第一播期Ｃ４９Ｓ）减数分型过程中有１７．９％的细胞发生异常，当小孢子从四分体释放后，大量细胞收缩变形，而使变异率急剧提高，以后小孢子的内含物逐渐解体，最终成为败育花粉，ⅠＣ４９Ｓ的花粉育性和结实率均很低。在ⅠＣ４９Ｓ小孢子败育过程中花药蛋白质含量低，被认为可能与花粉不育有关。而晚播（高温）的ⅥＣ４９Ｓ（第六播期Ｃ４９Ｓ）花粉发育基本正常，结实率较高。以Ｃ４９Ｓ为母本配的８个Ｆ１杂种结实率不同，最高达８０．５％，最低的仅１６．７％，表明不同基因型对Ｃ４９Ｓ不育性表达影响不同。
The staged sowing test of Wenling male-sterile wheat C49S showed that 17.9% of the cells in early-sowing (low-temperature) Ⅰ C49S (first sowing date C49S) After tetrad release, a large number of cells shrink and deform, resulting in a sharp increase in mutation rate. Afterwards, the contents of microspores gradually disintegrated and eventually become aborted pollen. The pollen fertility and seed setting rate of ⅠC49S was very low. The low protein content of anthers in the I C49S microspore abortion was considered to be related to pollen sterility. The late sowing (high temperature) VIC49S (sixth sowing date C49S) pollen development was normal, higher seed setting rate. Eight F1 hybrids with C49S as the female parent had different seed setting rates of up to 80.5% and lowest of only 16.7%, indicating that different genotypes had different effects on C49S sterility.