论文部分内容阅读
目的:建立高效液相色谱法,同时测定茚地那韦和依非韦伦在人血浆中的药物浓度。方法:色谱柱为岛津Shim-packCLC-ODS(6mm×15cm,5μm),流动相为乙腈-磷酸盐缓冲液(50:50),流速1.2mL.min-1,紫外检测波长:0～10min为260nm,10.01～25min为240nm。柱温为35℃。内标地西泮与茚地那韦和依非韦伦的保留时间分别为9.824,5.454,18.010min。结果:茚地那韦和依非韦伦线性范围分别为0.05～20mg.L-1(r=0.9990)和0.1～10mg.L-1(r=0.9996),最低检测限分别为0.05mg.L-1和0.1mg.L-1,样品溶液冻融稳定,日内精密度和日间精密度良好,回收率较高。结论:采用HPLC同时测定茚地那韦和依非韦伦血浆药物浓度,方法简便,结果准确可靠。
OBJECTIVE: To establish a HPLC method for the simultaneous determination of indinavir and efavirenz in human plasma. METHODS: Shimadzu Shim-pack CLC-ODS (6mm × 15cm, 5μm) was used as the mobile phase. The mobile phase consisted of acetonitrile-phosphate buffer (50:50), flow rate was 1.2mL.min-1, UV detection wavelength was 0-10min Was 260 nm, and 10.01-25 min was 240 nm. The column temperature was 35 ° C. Internal standard diazepam and indinavir and efavirenz retention times were 9.824,5.454,18.010min. Results: The linear range of indinavir and efavirenz were 0.05 ~ 20mg.L-1 (r = 0.9990) and 0.1 ~ 10mg.L-1 (r = 0.9996) .The detection limits were 0.05mg.L -1 and 0.1mg.L-1, the sample solution freeze-thaw stability, intra-day precision and good precision during the day, the recovery rate higher. Conclusion: Simultaneous determination of indinavir and efavirenz plasma concentration by HPLC, the method is simple and accurate, the results are accurate and reliable.