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目的观察黄绿青霉素(citreoviridin,CIT)对血管内皮细胞(vascular endothelial cells,VEC)的毒性及硒(selenium,Se)的保护作用。方法体外培养人脐静脉血管内皮细胞(human vascular endothelial cell,HUVEC),以亚硒酸钠(Na_2SeO_2)和黄绿青毒素(CIT)处理,分为:对照;Se(培养基含Se元素0.1mg/L);CIT低剂量(含0.2mg/L CIT);CIT高剂量(含0.4 mg/L CIT);Se+CIT(0.2mg/L Se和0.4mg/L CIT)5组。药物作用12 h后分别用MTT法检测细胞增殖活性,AnnexinV-FITC/PI双染法检测细胞凋亡,流式细胞仪检测细胞周期。结果 CIT组细胞增殖活性低于对照组,Se+CIT组细胞增殖活性高于CIT组(P<0.05);CIT组细胞凋亡率高于对照组,Se+CIT组细胞凋亡率低于CIT组(P<0.05);CIT组细胞发生G0/G1期细胞周期阻滞,Se+CIT组G0/G1期细胞周期阻滞不明显。结论 CIT可促进细胞凋亡,通过G0/G1期细胞周期的阻滞作用抑制HUVECs增殖活。
Objective To investigate the toxicity of citreoviridin (CIT) on the vascular endothelial cells (VECs) and the protective effect of selenium (Se). Methods Human umbilical vein endothelial cells (HUVECs) were cultured in vitro and treated with sodium selenite (Na2SeO_2) and yellow green toxin (CIT). The cells were divided into: control, Se ); CIT low dose (containing 0.2mg / L CIT); CIT high dose (containing 0.4mg / L CIT); Se + CIT (0.2mg / L Se and 0.4mg / L CIT) Cell viability was measured by MTT assay after 12 h of drug treatment. Apoptosis was detected by Annexin V-FITC / PI double staining and cell cycle was detected by flow cytometry. Results The cell proliferation activity in CIT group was lower than that in control group, and the cell proliferation activity in Se + CIT group was higher than that in CIT group (P <0.05). The apoptosis rate in CIT group was higher than that in control group, and the apoptosis rate in Se + CIT group was lower than that in CIT group (P <0.05). The cell cycle arrest in G0 / G1 phase in CIT group and the cell cycle arrest in G0 / G1 phase in Se + CIT group were not obvious. Conclusion CIT can promote cell apoptosis and inhibit the proliferation of HUVECs through the arrest of cell cycle in G0 / G1 phase.