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目的:探讨DNA修复基因甲基磺酸敏感性基因19(methyl methanesulfonate sensitivity gene19,MMS19)单核苷酸多态性与非小细胞肺癌(non-small cell lung carcer,NSCLC)组织学类型的关系。方法:收集原发性肺癌患者299例,应用AllGloTM探针结合实时荧光定量-PCR(real-time fluorogentic quantitative-PCR,RFQ-PCR)的方法分析MMS19基因rs3740526多态位点的基因型分布情况,采用卡方和回归方法比较不同基因型与NSCLC患者年龄、性别、是否吸烟以及肺癌组织学类型的关系。结果:MMS19(rs3740526)基因型在不同年龄、性别、吸烟群体中的分布,差异均无统计学意义(P值分别为0.609、0.621和0.755),在不同组织学类型(鳞癌和腺癌)中的分布,差异有统计学意义(P=0.026)。调整后比值比(odds ratio,OR)值分别为0.359[95%可信区间(confi dence interval,CI)=0.162～0.795,P=0.012]和0.445(95%CI=0.213～0.930,P=0.031)。结论:DNA修复基因MMS19rs3740526的多态性可能与NSCLC组织学类型具有相关性。
Objective: To investigate the relationship between single nucleotide polymorphisms of DNA methanesulfonate sensitivity gene19 (MMS19) and histological types of non-small cell lung carcer (NSCLC). Methods: A total of 299 patients with primary lung cancer were collected. Genotype distribution of rs3740526 polymorphism in MMS19 gene was analyzed by AllGloTM probe combined with real-time fluorogentic quantitative-PCR (RFQ-PCR) The relationship between different genotypes and age, sex, smoking status and histological type of lung cancer in NSCLC patients were compared using chi-square test and regression analysis. Results: There was no significant difference in the distribution of MMS19 (rs3740526) genotypes among different age, sex and smoking population (P = 0.609,0.621 and 0.755, respectively) In the distribution, the difference was statistically significant (P = 0.026). The adjusted odds ratio (OR) values were 0.359 [95% confidence interval (CI) = 0.162-0.795, P = 0.012] and 0.445 (95% CI = 0.213-0.930, P = 0.031 ). Conclusion: The polymorphism of DNA repair gene MMS19rs3740526 may be correlated with the histological type of NSCLC.