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用自制尼龙刷将培养至汇合的人脐静脉内皮细胞刮伤后,造成规则的内皮细胞缺失区。继续培养可见,原有的内皮钿胞很快迁移到缺失区,并分裂增殖。约48小时新生的内皮细胞即将缺失区全部修复而形成新的汇合单层。以DEAE-Sephacel离子交换及Sepharose 6B凝胶过滤柱层析分析损伤后修复的内皮细胞合成的蛋白聚糖时发现所合成的蛋白聚糖总量减少;硫酸乙酰肝素蛋白聚糖合成相对减少、而硫酸软骨素及/或硫酸皮肤素蛋白聚糖合成相对增多。说明:伴随着内皮细胞的损伤后修复其蛋白聚糖的合成也有质和量的改变。
Using a homemade nylon brush to confluent human umbilical vein endothelial cells scratch, resulting in a regular lack of endothelial cells. Continue to train visible, the original endothelial cells migrate quickly to the missing area, and proliferation. About 48 hours of newborn endothelial cells will be repaired completely to form a new confluent monolayer. The total amount of proteoglycan synthesized was reduced by DEAE-Sephacel ion exchange and Sepharose 6B gel filtration column chromatography on the proteoglycan synthesized by the endothelial cells after injury; the synthesis of heparan sulfate proteoglycan was relatively reduced, whereas Relative increase in the synthesis of chondroitin sulfate and / or dermatan sulfate proteoglycan. Explanation: There is also a qualitative and quantitative change in the synthesis of proteoglycans following endothelial cell injury.