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为研究人多发性骨髓瘤(Multiple Myeloma,MM)在SCID小鼠免疫重建前后体内生长的特 性和免疫细胞对肿瘤的作用,建立人多发性骨髓瘤(MM)荷瘤SCID小鼠模型。体外培养IL-6依 赖的人MM细胞株XC7,接种于SCID小鼠和用人淋巴细胞重建的SCID小鼠皮下;监测成瘤状态, 通过免疫组化观察细胞毒性T淋巴细胞(Cytotoxic T lymphocytes,CTLS)杀伤。结果表明:成 瘤后的肿瘤细胞CD28、CD138等分子的表达与接种前没有明显变化;免疫重建后的SCID小鼠成瘤 潜伏期延长,瘤体积小;免疫重建后的SCID小鼠肿瘤组织中,有人CD3+T细胞的浸润。结果提 示:SCID小鼠是建立肿瘤模型和肿瘤免疫研究的良好动物;免疫重建后的SCID小鼠体内肿瘤生 长受抑制;人MM细胞在小鼠体内的生长状态与淋巴细胞免疫有关。
To investigate the growth characteristics of human multiple myeloma (MM) in vivo and in vivo before and after immune reconstitution in SCID mice and the effect of immune cells on the tumor, a human multiple myeloma (MM) tumor bearing SCID mouse model was established. IL-6-dependent human MM cell line XC7 was cultured in vitro and subcutaneously in SCID mice and SCID mice reconstituted with human lymphocytes. The tumorigenesis status was monitored and the cytotoxic T lymphocytes (CTLSs) were observed by immunohistochemistry Kill. The results showed that the expression of CD28, CD138 and other molecules in the tumor cells after tumorigenesis did not change significantly before vaccination. The SCID mice after immunostimulation showed longer tumorigenicity and smaller tumor size. In the SCID mice, Infiltration of human CD3 + T cells. The results suggest that: SCID mice are good animal models for establishing tumor models and tumor immunity studies; tumor growth in SCID mice after immunosuppression is inhibited; and the growth status of human MM cells in mice is related to lymphocyte immunity.